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Temporary Research Technician

Job Description

Posting Number

PG194414TM

Position Number

11ASO3294

Position Type

Temporary

Essential Job Duties

The duties of this position is to culture E. coli to express plant lectin and then purify this plant protein with different chromatograph separations. The day to day duties include prepare LB medium, inoculation, solid and suspension cultures, extraction proteins, preparation of nickel columns, deae-sephadex ion exchange columns, and Sephadex columns, preparation of different equilibration, wash, and elution buffers, purification of proteins, preparation of SDS-PAGE gels, electrophoresis, imaging proteins, and data analysis. 

Is Time Limited

No

Job City & State

Raleigh, NC

Department

PMB

Classification Title

Temporary-Technical/Paraprofessional

Working Title

Temporary Research Technician

Work Schedule

20/hrs per week

Other Work/Responsibilities

  • n/a

Minimum Experience/Education

  • Combination of training and experience required to perform the
    duties

Department Required Skills

  • Experience with preparation of protein extraction and purification buffer, calibration of pH values and LB medium and autoclave
  • Experience with the growth and culture of E. coli
  • Experience with extraction of proteins 
  • Experience packing nickel column, equilibration, removal imidazole, and regeneration
  • Experience with Isolation of protein from nickel columns and precipitation with acetone or others agents
  • Experience preparing 12% SDS-PAGE gel and electrophoresis and imaging of proteins

Preferred Years Experience, Skills, Training, Education

  • n/a

Required License or Certification

  • n/a

Valid NC Driver's License required?

No

Commercial Driver's License Required?

No

Anticipated Hiring Range

$14-$17/hr

Job Open Date

05/24/2024

Quick Link

https://jobs.ncsu.edu/postings/202519

AA/EEO

NC State University is an equal opportunity and affirmative action employer. All qualified applicants will receive consideration for employment without regard to race, color, national origin, religion, sex, gender identity, age, sexual orientation, genetic information, status as an individual with a disability, or status as a protected veteran. Individuals with disabilities requiring disability-related accommodations in the application and interview process are welcome to contact 919-515-3148 to speak with a representative at the Office of Institutional Equity and Diversity.

If you have general questions about the application process, you may contact Human Resources at (919) 515-2135 or workatncstate@ncsu.edu.

Final candidates are subject to criminal & sex offender background checks. Some vacancies also require credit or motor vehicle checks. Degree(s) must be obtained prior to start date in order to meet qualifications and receive credit.

NC State University participates in E-Verify. Federal law requires all employers to verify the identity and employment eligibility of all persons hired to work in the United States.

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