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Duke University

Job Description

School of Medicine Established in 1930, Duke University School of Medicine is the youngest of the nation's top medical schools. Ranked sixth among medical schools in the nation, the School takes pride in being an inclusive community of outstanding learners, investigators, clinicians, and staff where interdisciplinary collaboration is embraced and great ideas accelerate translation of fundamental scientific discoveries to improve human health locally and around the globe. Composed of more than 2,500 faculty physicians and researchers, more than 1,300 students, and more than 6,000 staff, the Duke University School of Medicine along with the Duke University School of Nursing, Duke University Health System and the Private Diagnostic Clinic (PDC) comprise Duke Health. a world-class academic medical center. The Health System encompasses Duke University Hospital, Duke Regional Hospital, Duke Raleigh Hospital, Duke Primary Care, Duke Home and Hospice, Duke Health and Wellness, and multiple affiliations.

Occupational Summary 

The Asokan lab ( is affiliated with the Duke Department of Surgery, Molecular Genetics & Microbiology & Biomedical Engineering and is focused on the development and evaluation of novel gene therapy vectors. We are currently seeking to hire a motivated candidate for a Research Technician II position to assist with molecular biology techniques in the lab. A competitive salary and benefits package will be provided according to the Duke University School of Medicine guidelines.

Work Performed

The candidate will work closely with lab members including postdoctoral fellows, graduate students, research technicians, and is expected to independently perform daily activities which include, but may not be limited to:

  • Prepare culture media, chemicals, reagents and solutions
  • Carry out routine cell culture and perform mammalian cell transfections
  • Recombinant viral vector production
  • DNA/RNA extraction and cloning DNA constructs in bacterial systems.
  • Perform analytical techniques on vector preparations (qPCR, ELISA, protein gels etc).
  • Obtain, prepare, mount and stain tissue, blood and other substances for microscopic examination.
  • Perform recombinant protein expression from bacteria.
  • Ensure validity and reliability of data and maintain accurate records.
  • From time-to-time support additional high-priority projects to meet deadlines.
  • Attend and participate in relevant meetings, training, and development sessions.
  • Determine and setup instruments and operate laboratory equipment required for specific tests.
  • Use and maintain lab equipment in accordance with university’s health & safety guidelines.
  • Review laboratory methods manual, scientific journals, abstracts and other literature for information applicable to research experiments.
  • Review work of lower level laboratory personnel within designated areas and assist in training new personnel.

Preferred Minimum Qualifications

  • Bachelor’s degree in biology, chemistry, genetics, zoology, microbiology or other related scientific field.
  • Work requires two years of research experience.
  • Research experience involving mouse work is a plus.
  • Must be detail-oriented, very well organized, and approach laboratory procedures with critical thinking.
  • Strong initiative and eagerness to learn.
  • Outstanding problem-solving skills.
  • Must adhere to strict safety requirements.
  • Ability to work and communicate effectively in a dynamic group environment. 

Minimum Qualifications


Work generally requires a bachelor's degree in botany, biology, zoology, psychology or other directly related scientific field.


None required above education/training requirement. OR AN EQUIVALENT COMBINATION OF RELEVANT EDUCATION AND/OR EXPERIENCE

Job Level: 08

Duke is an Affirmative Action/Equal Opportunity Employer committed to providing employment opportunity without regard to an individual's age, color, disability, gender, gender expression, gender identity, genetic information, national origin, race, religion, sex, sexual orientation, or veteran status.

Duke aspires to create a community built on collaboration, innovation, creativity, and belonging. Our collective success depends on the robust exchange of ideas—an exchange that is best when the rich diversity of our perspectives, backgrounds, and experiences flourishes. To achieve this exchange, it is essential that all members of the community feel secure and welcome, that the contributions of all individuals are respected, and that all voices are heard. All members of our community have a responsibility to uphold these values.

Essential Physical Job Functions: Certain jobs at Duke University and Duke University Health System may include essential job functions that require specific physical and/or mental abilities. Additional information and provision for requests for reasonable accommodation will be provided by each hiring department.

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