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RESEARCH TECHNICIAN II
School of Medicine Established in 1930, Duke University School of Medicine is the youngest of the nation's top medical schools. Ranked sixth among medical schools in the nation, the School takes pride in being an inclusive community of outstanding learners, investigators, clinicians, and staff where interdisciplinary collaboration is embraced and great ideas accelerate translation of fundamental scientific discoveries to improve human health locally and around the globe. Composed of more than 2,500 faculty physicians and researchers, more than 1,300 students, and more than 6,000 staff, the Duke University School of Medicine along with the Duke University School of Nursing, Duke University Health System and the Private Diagnostic Clinic (PDC) comprise Duke Health. a world-class academic medical center. The Health System encompasses Duke University Hospital, Duke Regional Hospital, Duke Raleigh Hospital, Duke Primary Care, Duke Home and Hospice, Duke Health and Wellness, and multiple affiliations.
The Asokan lab (www.asokanlab.org) is affiliated with the Duke Department of Surgery, Molecular Genetics & Microbiology & Biomedical Engineering and is focused on the development and evaluation of novel gene therapy vectors. We are currently seeking to hire a motivated candidate for a Research Technician II position to assist with molecular biology and in vivo murine studies in the lab. A competitive salary and benefits package will be provided according to the Duke University School of Medicine guidelines.
The candidate will work closely with lab members including postdoctoral fellows, graduate students, research technicians, and is expected to independently perform daily activities which include, but may not be limited to:
- Maintain mouse colony management including:
- Record-keeping and inventory of the colony
- Weaning, breeding, and monitoring mice
- Collection and preparation of genotyping samples
- Perform a wide range of dosing and procedures in mouse models
- Obtain, prepare, mount and stain tissue, blood and other substances for microscopic examination.
- Assist with tissue harvests and processing; techniques include cryosectioning, immunohistochemistry, immunofluorescence, and other histology methods
- Experience with molecular biology techniques including: DNA/RNA extraction, PCR/qPCR, ELISA and Western blot
- Compile, document, and maintain precise records of experimental work
- Coordinate in vivo experiments with collaborators and industrial partners
- Drafting IACUC protocols, amendments, and renewals, and ensuring compliance with IACUC guidelines
- Operate laboratory instruments and equipment required for analysis, histology, microscopy, and Flow cytometry
- Assist in training new personnel.
- Review laboratory methods manual, scientific journals, abstracts and other literature for information applicable to research experiments.
- Attend and participate in relevant meetings, training, and development sessions.
- Perform other related duties to the work described herein
- Work requires a Bachelor’s degree in biology, genetics, zoology, microbiology or related scientific field.
- Must be detail-oriented, well organized
- Strong initiative and eagerness to learn
- Must adhere to strict lab safety requirements
- Ability to work and communicate effectively in a dynamic group environment.
- Previous experience with mouse handling and colony maintenance is required. Prior experience with molecular biology techniques, mouse injections and/or tissue processing is preferred.
Work generally requires a bachelor's degree in botany, biology, zoology, psychology or other directly related scientific field.
None required above education/training requirement. OR AN EQUIVALENT COMBINATION OF RELEVANT EDUCATION AND/OR EXPERIENCE
Job Code: 00000951 RESEARCH TECHNICIAN II
Job Level: 08
Duke is an Affirmative Action/Equal Opportunity Employer committed to providing employment opportunity without regard to an individual's age, color, disability, gender, gender expression, gender identity, genetic information, national origin, race, religion, sex, sexual orientation, or veteran status.
Duke aspires to create a community built on collaboration, innovation, creativity, and belonging. Our collective success depends on the robust exchange of ideas—an exchange that is best when the rich diversity of our perspectives, backgrounds, and experiences flourishes. To achieve this exchange, it is essential that all members of the community feel secure and welcome, that the contributions of all individuals are respected, and that all voices are heard. All members of our community have a responsibility to uphold these values.
Essential Physical Job Functions: Certain jobs at Duke University and Duke University Health System may include essential job functions that require specific physical and/or mental abilities. Additional information and provision for requests for reasonable accommodation will be provided by each hiring department.
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